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Saddleback College Real Time Quantitative PCR Discussion

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I’m working on a biochemistry exercise and need a sample draft to help me learn.

qPCR is a complex technique with many steps. Read the description and watch the movies provided below prior to watching the lab demonstration video at the bottom of this page.

  • Diagram of the qPCR workflow:

real-time-qpcr-workflow.jpg

  • qPCR Analysis Video (10:07 – watch until 6:20 to learn about absolute quantification)

there is the main video