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UF MOD 1 The Total Number of Loci Science Discussion

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Consider the following electropherograms from the controls and ladder in your case study sample set.

Ladder

Positive Control

Negative Control

1. How many loci are present in the amplification kit being used for this case study? 

2. What is the benefit of having 10 mini-STR loci in this kit? 

3. What is the role of the orange peaks present in these samples? 

4. What is the role of the ladder? 

5.  You are being presented with data that has not been analyzed yet to determine what peaks are non-allelic peaks in your positive control.  Identify the true DNA peaks belonging to the control DNA and identify the peaks that are artifacts from the amplification or capillary electrophoresis process. Record your results in the Module 1 Allele Call Worksheet   [10]